Coding

Part:BBa_J70601:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)

VioC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgaaacgtgcgattatcgttggtgg l: 54 P(g/b): g Ho(max#):-112.47 Het(max#):-112.88 Tm: 69.7 GC(%): 50

Reverse primer: gtttcttcctgcagcggccgctactagtattattaattcacgcgaccaatcttgtacc l: 58 P(g/b): g Ho(max#): -112.8 Het(max#):-112.88 Tm: 68.5 GC(%): 46.6

Source

designed

References